3E0S
Crystal structure of an uncharacterized protein from Chlorobium tepidum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-08 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.740, 212.477, 55.424 |
| Unit cell angles | 90.00, 106.94, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.090 |
| R-factor | 0.221 |
| Rwork | 0.218 |
| R-free | 0.27200 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.331 |
| Data reduction software | DENZO |
| Data scaling software | SCALA (3.2.19) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.529 | 2.200 |
| High resolution limit [Å] | 2.089 | 2.090 |
| Rmerge | 0.089 | 0.325 |
| Total number of observations | 19090 | |
| Number of reflections | 40989 | |
| <I/σ(I)> | 12.2 | 3.9 |
| Completeness [%] | 93.2 | 85.6 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 13% PEG 8K, 400mM lithium sulfate, pH 7.0, Vapor diffusion, temperature 294K |
