3E0R
Crystal structure of cppA protein from Streptococcus pneumoniae TIGR4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-09 |
Detector | SBC-3 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 114.742, 130.741, 91.535 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.300 |
R-factor | 0.19277 |
Rwork | 0.189 |
R-free | 0.25632 |
Structure solution method | SAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.758 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.151 | 0.555 |
Number of reflections | 62250 | |
<I/σ(I)> | 9.5 | 2 |
Completeness [%] | 97.7 | 83.4 |
Redundancy | 4.4 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 0.01M sodium citrate 33% PEG 6000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |