3E0O
Crystal structure of MsrB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97182 |
| Spacegroup name | P 3 |
| Unit cell lengths | 136.114, 136.114, 61.925 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.790 - 2.600 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.29400 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.800 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.101 | 0.436 |
| Number of reflections | 39083 | |
| <I/σ(I)> | 12.5 | 2.8 |
| Completeness [%] | 99.4 | 97.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.25 | 298 | 1.2M Lithium sulfate, 0.1M HEPES, pH 7.25, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.25 | 298 | 1.2M Lithium sulfate, 0.1M HEPES, pH 7.25, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
