3E0M
Crystal structure of fusion protein of MsrA and MsrB
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-14 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97950, 0.97962, 0.97182 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 158.521, 165.489, 77.281 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.990 - 2.400 |
R-factor | 0.239 |
Rwork | 0.239 |
R-free | 0.28200 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.057 | 0.279 |
Number of reflections | 77162 | |
<I/σ(I)> | 37.9 | 2.6 |
Completeness [%] | 91.2 | 49.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | 0.1M MES (pH 5.6), 1% (w/v) PEG 4000, 0.2M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | 0.1M MES (pH 5.6), 1% (w/v) PEG 4000, 0.2M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |