3E0E

Crystal structure of a domain of replication protein A from Methanococcus maripaludis. NorthEast Structural Genomics targe MrR110B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-29
Detector	ADSC QUANTUM 210
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	25.263, 54.896, 56.223
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.280 - 1.600
R-factor	0.234
Rwork	0.234
R-free	0.26900
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.048	0.225
Number of reflections	19963
<I/σ(I)>		19.5
Completeness [%]	99.7	98.7
Redundancy	21.4	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5	293	Sodium chloride 0.1 M, Sodium Acetate 0.1 M, PEG 4000 40%, pH 5, Micro batch under oil method, temperature 293K
1		5	293	Sodium chloride 0.1 M, Sodium Acetate 0.1 M, PEG 4000 40%, pH 5, Micro batch under oil method, temperature 293K