3DZW
Structure of Narcissus pseudonarcissus lectin complex with Mannobiose at 1.7 A resolution, form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07-01 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.003, 102.011, 74.366 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.031 - 1.700 |
| R-factor | 0.18823 |
| Rwork | 0.187 |
| R-free | 0.21279 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1npl |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.544 |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.031 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.077 | 0.382 |
| Number of reflections | 30200 | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 293 | 10 mg/ml in Phosphate buffered saline, containing up to 20mM beta-1,3 mannobiose, pH 8.0, VAPOR DIFFUSION, temperature 293K |
