3DZK
Crystal structure of human CD38 extracellular domain, NMN complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-01 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 1 |
Unit cell lengths | 41.889, 53.205, 65.598 |
Unit cell angles | 106.20, 91.84, 95.05 |
Refinement procedure
Resolution | 20.000 - 1.810 |
R-factor | 0.176 |
Rwork | 0.174 |
R-free | 0.21700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yh3 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.523 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.3.0021) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.099 | 0.443 |
Number of reflections | 47229 | |
<I/σ(I)> | 12.6 | 1.5 |
Completeness [%] | 96.7 | 87.2 |
Redundancy | 3.4 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 298 | 100 mM MES pH 6.0, 15% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |