3DZE
Crystal structure of bovine coupling Factor B bound with cadmium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-12 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0332 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.010, 49.924, 36.942 |
Unit cell angles | 90.00, 109.58, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.150 |
R-factor | 0.1305 |
Rwork | 0.129 |
R-free | 0.15496 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.035 |
RMSD bond angle | 2.322 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.140 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.036 | 0.304 |
Number of reflections | 62817 | |
<I/σ(I)> | 32.2 | 1.84 |
Completeness [%] | 83.5 | 11.3 |
Redundancy | 3.3 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | 50% PPG 400, 100 mM Tris, pH 7.4-8.0, vapor diffusion, temperature 277K |