3DZD
Crystal structure of sigma54 activator NTRC4 in the inactive state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.28237 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.897, 93.105, 114.163 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.040 - 2.400 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ny5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.961 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.520 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.055 | 0.496 |
| Number of reflections | 37966 | |
| <I/σ(I)> | 22.6 | 2 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 3.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 298 | 5% PEG 4000, 150mM NaCl, 50mM Citric Acid, 5% Glycerol, pH 4.6, vapor diffusion, hanging drop, temperature 298K |
