3DYE
Crystal structure of AED7-norepineprhine complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-17 |
| Detector | SBC CCD |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.879, 65.993, 52.764 |
| Unit cell angles | 90.00, 113.48, 90.00 |
Refinement procedure
| Resolution | 27.270 - 1.750 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.23100 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.016 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.820 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.068 | 0.328 |
| Number of reflections | 31957 | |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 99.6 | 97 |
| Redundancy | 4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 16-20% PEG 6000, Tris HCl, 0.1 M, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
