3DYC

Structure of E322Y Alkaline Phosphatase in Complex with Inorganic Phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-08
Detector	ADSC QUANTUM 315
Wavelength(s)	1.2825
Spacegroup name	P 63 2 2
Unit cell lengths	161.609, 161.609, 139.266
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.452 - 2.303
R-factor	0.184
Rwork	0.181
R-free	0.24300
RMSD bond length	0.006
RMSD bond angle	1.000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Rmerge	0.174
Number of reflections	86186
Completeness [%]	95.6
Redundancy	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.2 M ammonium fluoride, 20% PEG 3350, and 0.5 mM zinc chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K