3DXZ
Crystal structure of EcTrmB in complex with SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-22 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.570, 48.290, 57.480 |
Unit cell angles | 90.00, 90.52, 90.00 |
Refinement procedure
Resolution | 19.450 - 1.580 |
R-factor | 0.17517 |
Rwork | 0.174 |
R-free | 0.19285 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dxx |
RMSD bond length | 0.007 |
RMSD bond angle | 1.158 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.970 |
High resolution limit [Å] | 1.580 |
Number of reflections | 34161 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 283 | 15% PEG 3350 (w/v), 150 mM MgSO4, VAPOR DIFFUSION, HANGING DROP, temperature 283K |