3DXL
Crystal structure of AeD7 from Aedes Aegypti
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Collection date | 2006-12-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.892, 65.477, 52.047 |
| Unit cell angles | 90.00, 112.74, 90.00 |
Refinement procedure
| Resolution | 27.050 - 1.300 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.21400 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.085 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.089 | 0.304 |
| Number of reflections | 75797 | |
| <I/σ(I)> | 15.4 | 5.3 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 17-20 % PEG 6000, 100 mM Tris HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
