3DXH
Ribonuclease A uridine 5' diphosphate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-17 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0448 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.003, 32.337, 72.299 |
| Unit cell angles | 90.00, 90.72, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.400 |
| R-factor | 0.18976 |
| Rwork | 0.188 |
| R-free | 0.22497 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2g8q |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.410 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 42757 | |
| <I/σ(I)> | 29 | 8.2 |
| Completeness [%] | 97.7 | 100 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | PEG 4000, sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
