3DX2
Golgi mannosidase II complex with MANNOSTATIN B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.977 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.630, 109.281, 137.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.900 - 1.400 |
R-factor | 0.142 |
Rwork | 0.142 |
R-free | 0.16400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hty |
RMSD bond length | 0.012 |
RMSD bond angle | 1.465 |
Data reduction software | HKL-2000 |
Data scaling software | SADABS |
Phasing software | CNS |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.050 | 0.290 |
Number of reflections | 196104 | |
<I/σ(I)> | 17.3 | 3.8 |
Completeness [%] | 96.3 | 73.8 |
Redundancy | 4.9 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG8000, Tris, 2.5% MPD, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |