3DWP
Crystal structure of the B-subunit of the AB5 toxin from E. Coli with Neu5Gc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Spacegroup name | P 65 |
| Unit cell lengths | 96.900, 96.900, 165.622 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.970 - 2.200 |
| R-factor | 0.19081 |
| Rwork | 0.188 |
| R-free | 0.23992 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.468 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.920 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 39693 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 16% PEG 3350, 100mM sodium cacodylate, 200mM ammonium fluoride, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
