3DWG

Crystal structure of a sulfur carrier protein complex found in the cysteine biosynthetic pathway of Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2005-08-22
Detector	ADSC QUANTUM 315
Spacegroup name	P 1 21 1
Unit cell lengths	55.810, 80.426, 89.619
Unit cell angles	90.00, 105.74, 90.00

Refinement procedure

Resolution	27.130 - 1.530
R-factor	0.178
Rwork	0.176
R-free	0.21700
Structure solution method	SAD
RMSD bond length	0.020
RMSD bond angle	1.899
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.570
High resolution limit [Å]	1.530	1.530
Number of reflections	105098
<I/σ(I)>	26.2	4
Completeness [%]	96.3	81.6
Redundancy	3.18	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.8	295	7-10% w/v PEG 4000, 0.1 M Sodium citrate pH 5.8, 0.2 M Ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 295K, pH 5.80
1		5.8	295	7-10% w/v PEG 4000, 0.1 M Sodium citrate pH 5.8, 0.2 M Ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 295K, pH 5.80
1		5.8	295	7-10% w/v PEG 4000, 0.1 M Sodium citrate pH 5.8, 0.2 M Ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 295K, pH 5.80