3DWD

Crystal structure of the ArfGAP domain of human ARFGAP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97935
Spacegroup name	P 6
Unit cell lengths	82.422, 82.422, 81.008
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.236
Rwork	0.234
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gf9
RMSD bond length	0.017
RMSD bond angle	1.445
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0044)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.092	0.037	0.653
Number of reflections	12298
<I/σ(I)>	8.1
Completeness [%]	99.6	97.4	98.3
Redundancy	7.4	7.3	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	3.5M Sodium Formate, 0.1M Tris, 1:100 chymotrypsin, pH 8.5, vapor diffusion, sitting drop, temperature 291K