3DWA
Crystal structure of the B-subunit of the AB5 toxin from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Spacegroup name | P 65 |
Unit cell lengths | 97.630, 97.630, 165.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.800 - 2.084 |
R-factor | 0.19182 |
Rwork | 0.189 |
R-free | 0.23626 |
Structure solution method | SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.702 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.220 | |
High resolution limit [Å] | 2.084 | 2.084 |
Number of reflections | 45985 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 16%(w/v) PEG 3350, 100mM sodium cacodylate, 200mM ammonium fluoride, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |