3DW4
Crystal Structure of the Sarcin/Ricin Domain from E. COLI 23 S rRNA, U2650-OCH3 modified
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2008-04-22 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.85 |
| Spacegroup name | P 43 |
| Unit cell lengths | 29.630, 29.630, 76.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.622 - 0.970 |
| R-factor | 0.139 |
| Rwork | 0.139 |
| R-free | 0.15000 |
| Starting model (for MR) | 1q9a |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.173 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 0.980 | |
| High resolution limit [Å] | 0.970 | 0.970 |
| Number of reflections | 38820 | 1194 |
| <I/σ(I)> | 31.95 | 3.4 |
| Completeness [%] | 99.9 | 98.6 |
| Redundancy | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 3.2 M (NH4)2SO4, 50 mM K-MOPS pH 7.0, 10 mM MgCl2, 10 mM MnCl2, vapor diffusion, hanging drop, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | (NH4)2SO4 | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | K-MOPS | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | MnCl2 | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | (NH4)2SO4 | ||
| 7 | 1 | 2 | K-MOPS | ||
| 8 | 1 | 2 | MgCl2 | ||
| 9 | 1 | 2 | MnCl2 |
