Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 77 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-24 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.300, 91.300, 73.100 |
| Unit cell angles | 90.00, 112.60, 90.00 |
Refinement procedure
| Resolution | 19.760 - 1.600 |
| R-factor | 0.18875 |
| Rwork | 0.188 |
| R-free | 0.21080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dy6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.255 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.760 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 86680 | |
| <I/σ(I)> | 21.54 | 11.68 |
| Completeness [%] | 96.5 | 95.2 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 20% PEG 6000, 0.1 M KSCN, 0.1 M Sodium Acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
