3DVT
Biochemical and structural characterization of the PAK1- LC8 interaction
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 |
Unit cell lengths | 36.482, 44.871, 84.825 |
Unit cell angles | 79.62, 77.54, 88.03 |
Refinement procedure
Resolution | 27.780 - 2.300 |
R-factor | 0.19263 |
Rwork | 0.189 |
R-free | 0.25770 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.501 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0034) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 22187 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 50 mM MgCl2, 100 mM MES, 32% (w/v) PEG 4000, and 20% xylitol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |