3DVO
SgrAI with cognate DNA and calcium bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.136, 119.248, 123.799 |
Unit cell angles | 90.00, 95.62, 90.00 |
Refinement procedure
Resolution | 41.068 - 1.892 |
R-factor | 0.2211 |
Rwork | 0.220 |
R-free | 0.27320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dpg |
RMSD bond length | 0.008 |
RMSD bond angle | 1.255 |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.890 |
Rmerge | 0.120 |
Number of reflections | 126080 |
<I/σ(I)> | 6.5 |
Completeness [%] | 92.8 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | PEG4000, buffer, NaCl, CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG4000 | ||
2 | 1 | 1 | NaCl | ||
3 | 1 | 1 | CaCl2 | ||
4 | 1 | 2 | PEG4000 | ||
5 | 1 | 2 | NaCl | ||
6 | 1 | 2 | CaCl2 |