3DVM

Crystal Structure of Ca2+/CaM-CaV2.1 IQ domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-22
Detector	ADSC QUANTUM 315
Wavelength(s)	1.11587
Spacegroup name	P 61 2 2
Unit cell lengths	43.956, 43.956, 337.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.278
Rwork	0.276
R-free	0.32000
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.353
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.13)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.055	0.272
Number of reflections	6481
Completeness [%]	94.7	72.3
Redundancy	16.4	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	292	0.1 M Bis-Tris, 25 % PEG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K
1	VAPOR DIFFUSION, HANGING DROP	5.5	292	0.1 M Bis-Tris, 25 % PEG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K
1	VAPOR DIFFUSION, HANGING DROP	5.5	292	0.1 M Bis-Tris, 25 % PEG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K
1	VAPOR DIFFUSION, HANGING DROP	5.5	292	0.1 M Bis-Tris, 25 % PEG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K
1	VAPOR DIFFUSION, HANGING DROP	5.5	292	0.1 M Bis-Tris, 25 % PEG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K