3DVH
LC8 Point mutant K36P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 163.033, 37.951, 44.873 |
| Unit cell angles | 90.00, 100.92, 90.00 |
Refinement procedure
| Resolution | 27.200 - 2.000 |
| R-factor | 0.20368 |
| Rwork | 0.201 |
| R-free | 0.24382 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2PG1 monomer A |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.689 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.900 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 18462 | |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 50 mM CaCl2, 100 mM HEPES, 28% (w/v) PEG MME 2000, 10% glycerol, 10 mM DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
