3DV8
Crystal structure of a putative transcriptional regulator of the crp/fnr family (eubrec_1222) from eubacterium rectale atcc 33656 at 2.55 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-15 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 45.560, 88.482, 181.304 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.237 - 2.550 |
R-factor | 0.224 |
Rwork | 0.222 |
R-free | 0.26200 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.566 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.237 | 44.237 | 2.640 |
High resolution limit [Å] | 2.550 | 5.460 | 2.550 |
Rmerge | 0.123 | 0.067 | 0.728 |
Number of reflections | 12310 | 1348 | 1136 |
<I/σ(I)> | 7.24 | 13.6 | 1.9 |
Completeness [%] | 99.3 | 98.5 | 95.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.14 | 293 | 5.0% polyethylene glycol 8000, 20.0% polyethylene glycol 300, 10.0% Glycerol, 0.1M TRIS pH 8.14, NANODROP', VAPOR DIFFUSION, SITTING DROP, temperature 293K |