3DUZ
Crystal structure of the postfusion form of baculovirus fusion protein GP64
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0039, 0.8856, 1.0714, 1.0711 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 87.470, 87.470, 431.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.840 - 2.950 |
| R-factor | 0.22606 |
| Rwork | 0.224 |
| R-free | 0.26960 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.087 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.110 | 0.550 |
| Number of reflections | 13594 | |
| <I/σ(I)> | 16.3 | 3.29 |
| Completeness [%] | 97.3 | 99.9 |
| Redundancy | 6.8 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 10% PEG 6000, 100 mM citric acid, 200 mM potassium sodium tartrate, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
