3DUK

CRYSTAL STRUCTURE OF A NTF2-LIKE PROTEIN OF UNKNOWN FUNCTION (MFLA_0564) FROM METHYLOBACILLUS FLAGELLATUS KT AT 2.200 A RESOLUTION

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-01
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97932,0.97911
Spacegroup name	P 32 2 1
Unit cell lengths	103.030, 103.030, 130.950
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.981 - 2.200
R-factor	0.1653
Rwork	0.163
R-free	0.21170
Structure solution method	MAD
RMSD bond length	0.003
RMSD bond angle	0.572
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.981	29.980	2.280
High resolution limit [Å]	2.200	4.720	2.200
Rmerge		0.031	0.592
Number of reflections	41254
<I/σ(I)>	12.85	35.4	2.6
Completeness [%]	99.6	99.3	97.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	20.0% 2-propanol, 30.0% polyethylene glycol 4000, 0.1M citric acid pH 5.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K, VAPOR DIFFUSION, SITTING DROP