3DUI
Crystal structure of the oxidized CG-1B: an adhesion/growth-regulatory lectin from chicken
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 71.590, 107.640, 35.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.350 - 2.100 |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qmj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.350 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.063 | 0.244 |
| Number of reflections | 16792 | |
| <I/σ(I)> | 6.9 | 2.9 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 5 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 30% (v/v) PEG 8000, 1% beta-mercaptoethanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
