3DUG
Crystal structure of zn-dependent arginine carboxypeptidase complexed with zinc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-21 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 113.325, 146.040, 255.646 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.620 |
| R-factor | 0.23106 |
| Rwork | 0.230 |
| R-free | 0.26364 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3be7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.175 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.710 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.110 | 0.790 |
| Number of reflections | 127892 | |
| <I/σ(I)> | 4.1 | 0.7 |
| Completeness [%] | 99.4 | 98.4 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 100MM SUCCINIC ACID PH 7.0, 15% PEG 3350, 5MM ZINC SULFATE, 10% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, REMARK TEMPERATURE 294K, pH 7.00 |
