3DU1
The 2.0 Angstrom Resolution Crystal Structure of HetL, a Pentapeptide Repeat Protein involved in Heterocyst Differentiation Regulation from the Cyanobacterium Nostoc sp. Strain PCC 7120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-13 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 68.200, 93.763, 101.307 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.410 - 2.000 |
| R-factor | 0.1773 |
| Rwork | 0.176 |
| R-free | 0.20420 |
| Structure solution method | sulfur anomalous scattering |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.344 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.840 | 1.870 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.300 | |
| Number of reflections | 28767 | |
| <I/σ(I)> | 61.22 | 4.63 |
| Completeness [%] | 89.9 | 58.3 |
| Redundancy | 38.58 | 38.58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1M HEPES, 0.7M sodium phosphate monobasic, 0.7M potassium phosphate monobasic, 12.5% glycerol, protein concentration 7.5 mg/ml, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
