3DU0
E. coli dihydrodipicolinate synthase with first substrate, pyruvate, bound in active site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Detector technology | IMAGE PLATE |
Collection date | 2005-09-15 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 120.950, 120.950, 110.030 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.140 - 2.000 |
R-factor | 0.191 |
Rwork | 0.188 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.221 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 32.140 |
High resolution limit [Å] | 2.000 |
Rmerge | 0.083 |
Number of reflections | 62822 |
<I/σ(I)> | 10.4 |
Completeness [%] | 99.6 |
Redundancy | 4.08 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 10 | 277 | 1.8M K2HPO4, N-octyl-beta-(R)-glucopyranoside, pH 10, hanging drop, temperature 277K |