3DU0
E. coli dihydrodipicolinate synthase with first substrate, pyruvate, bound in active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-09-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 120.950, 120.950, 110.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.140 - 2.000 |
| R-factor | 0.191 |
| Rwork | 0.188 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.221 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.140 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.083 |
| Number of reflections | 62822 |
| <I/σ(I)> | 10.4 |
| Completeness [%] | 99.6 |
| Redundancy | 4.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 10 | 277 | 1.8M K2HPO4, N-octyl-beta-(R)-glucopyranoside, pH 10, hanging drop, temperature 277K |
