3DTK
Crystal structure of the IRRE protein, a central regulator of DNA damage repair in deinococcaceae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.93300 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 87.260, 52.672, 63.221 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.830 - 3.240 |
R-factor | 0.22853 |
Rwork | 0.226 |
R-free | 0.27702 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dte |
RMSD bond length | 0.007 |
RMSD bond angle | 0.867 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.420 |
High resolution limit [Å] | 3.240 | 3.240 |
Number of reflections | 4827 | |
<I/σ(I)> | 13.1 | 2.2 |
Completeness [%] | 97.7 | 97.9 |
Redundancy | 4.5 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 281 | 20% PEG 3350, 0.2M Potassium fluoride, pH 7.5, VAPOR DIFFUSION, temperature 281K |