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3DTA

E(L212)Q, N(M44)D double mutant structure of photosynthetic reaction center from Rhodobacter sphaeroides

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RU200
Temperature [K]293
Detector technologyIMAGE PLATE
Collection date2004-10-15
DetectorRIGAKU RAXIS IIC
Wavelength(s)1.54
Spacegroup nameP 31 2 1
Unit cell lengths141.500, 141.500, 187.000
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution30.000 - 3.200
Rwork0.199
R-free0.20290
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1k6l
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0003.310
High resolution limit [Å]3.2003.200
Rmerge0.1330.404
Number of reflections32667
<I/σ(I)>9.32.6
Completeness [%]95.096
Redundancy2.52.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7.5293Potassium phosphate, LDAO, Heptane triol, Dioxane, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

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