3DT5
C_terminal domain of protein of unknown function AF_0924 from Archaeoglobus fulgidus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 65.413, 65.413, 76.582 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.000 - 1.940 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.20200 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.631 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.063 | 0.881 |
| Number of reflections | 12898 | |
| <I/σ(I)> | 13 | 4.18 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 27.6 | 23.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.2 M calcium chloride, 25% PEG 5500 MME, 0.1 M Bis-Tris buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
