3DSX
Crystal structure of RabGGTase(DELTA LRR; DELTA IG)in complex with di-prenylated peptide Ser-Cys(GG)-Ser-Cys(GG) derivated from Rab7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-27 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9841 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.354, 91.153, 114.591 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.370 - 2.100 |
R-factor | 0.17056 |
Rwork | 0.168 |
R-free | 0.22942 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | DB ENTRY 3DSS |
RMSD bond length | 0.011 |
RMSD bond angle | 1.285 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 41826 | |
<I/σ(I)> | 23.4 | 6.9 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.2 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 284 | 14% (w/v) PEG 3350, 0.2M Ca(OAc)2, 2.5% (v/v)DMSO, 0.1M HEPES, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 284K |