3DSW
Crystal structure of RabGGTase(DELTA LRR; DELTA IG)in complex with mono-prenylated peptide Ser-Cys(GG)-Ser-Cys derivated from Rab7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-09-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9825 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.051, 90.908, 114.343 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.210 - 2.150 |
R-factor | 0.17278 |
Rwork | 0.170 |
R-free | 0.23154 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dss |
RMSD bond length | 0.012 |
RMSD bond angle | 1.446 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.400 | 2.250 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 38414 | |
<I/σ(I)> | 15.2 | 5.2 |
Completeness [%] | 99.1 | 99.8 |
Redundancy | 4 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 284 | 14% (w/v) PEG 3350, 0.2M Ca(OAc)2, 0.1M HEPES, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 284K |