3DSP
Crystal structure of apo copper resistance protein CopK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-11-22 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.760, 37.510, 27.650 |
| Unit cell angles | 90.00, 103.69, 90.00 |
Refinement procedure
| Resolution | 34.140 - 2.200 |
| R-factor | 0.223 |
| Rwork | 0.221 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dso |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.521 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.050 | 0.252 |
| Number of reflections | 4293 | |
| <I/σ(I)> | 26.1 | 5.35 |
| Completeness [%] | 97.7 | 94.8 |
| Redundancy | 8.6 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 298 | 1.4M Sodium citrate, 0.1M Na-HEPES, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
