3DSO

Crystal structure of Cu(I) bound copper resistance protein CopK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-06-13
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54179
Spacegroup name	P 21 21 2
Unit cell lengths	32.193, 84.672, 23.662
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.800 - 1.550
R-factor	0.203
Rwork	0.203
R-free	0.21200
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.582
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.610
High resolution limit [Å]	1.550	3.340	1.550
Rmerge	0.043	0.042	0.129
Rmeas	0.043
Total number of observations	87158
Number of reflections	9911
<I/σ(I)>	27.1		13.5
Completeness [%]	99.1	98.2	93.7
Redundancy	8.8	8.8	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1M KSCN, 32% PEG MME 2000, VAPOR DIFFUSION, HANGING DROP, temperature 298K