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3DSN

Crystal structure of the complex of the Caf1M chaperone with the mini-fiber of two Caf1 subunits (Caf1:Caf1), carrying the Thr7Phe mutation in the Gd donor strand

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID14-2
Synchrotron siteESRF
BeamlineID14-2
Temperature [K]100
Detector technologyCCD
Collection date2005-12-16
DetectorADSC QUANTUM 4
Wavelength(s)0.933
Spacegroup nameP 21 21 21
Unit cell lengths71.690, 91.149, 165.483
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution20.000 - 2.200
R-factor0.23677
Rwork0.234
R-free0.28138
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1z9s
RMSD bond length0.012
RMSD bond angle1.396
Data reduction softwareMOSFLM
Data scaling softwareSCALA
Phasing softwareMOLREP
Refinement softwareREFMAC (5.2.0005)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]55.0002.320
High resolution limit [Å]2.2002.200
Rmerge0.0990.398
Number of reflections55891
<I/σ(I)>11.82.9
Completeness [%]100.0100
Redundancy3.63.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.929316-17% PEG 8000 in 0.1 M Na cacodylate and 0.2 M Ca acetate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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