3DSG
XC1028 from Xanthomonas campestris Adopts a PilZ Domain-like Structure Yet with Trivial c-di-GMP Binding Activity
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-01-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97920, 0.96398, 0.97888 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 87.896, 50.962, 92.745 |
Unit cell angles | 90.00, 90.21, 90.00 |
Refinement procedure
Resolution | 25.480 - 2.090 |
R-factor | 0.277 |
Rwork | 0.277 |
R-free | 0.27800 |
Structure solution method | MAD |
RMSD bond length | 0.040 |
RMSD bond angle | 4.800 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.180 |
High resolution limit [Å] | 2.090 | 2.100 |
Rmerge | 0.044 | 0.220 |
Number of reflections | 22930 | |
<I/σ(I)> | 27.3 | 5.3 |
Completeness [%] | 94.7 | 79 |
Redundancy | 5.7 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 297 | 0.1M sodium cacodylate pH 6.5, 18% PEG 2KMME, 2% glycerol, VAPOR DIFFUSION, temperature 297K |