3DS8
The crystal structure of the gene lin2722 products from Listeria innocua
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 63.211, 63.211, 163.075 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.940 - 1.800 |
| R-factor | 0.16973 |
| Rwork | 0.168 |
| R-free | 0.20373 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.656 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.940 | 1.847 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.108 | 0.580 |
| Number of reflections | 29792 | |
| <I/σ(I)> | 31.68 | 2.1 |
| Completeness [%] | 99.3 | 95.29 |
| Redundancy | 17 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 10% Glycerol, 20.5% PEK4000, 0.065M tris_Na Citrate, 0.1M NH4 acetate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
