3DS8
The crystal structure of the gene lin2722 products from Listeria innocua
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9796 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 63.211, 63.211, 163.075 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.940 - 1.800 |
R-factor | 0.16973 |
Rwork | 0.168 |
R-free | 0.20373 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.656 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.940 | 1.847 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.108 | 0.580 |
Number of reflections | 29792 | |
<I/σ(I)> | 31.68 | 2.1 |
Completeness [%] | 99.3 | 95.29 |
Redundancy | 17 | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 10% Glycerol, 20.5% PEK4000, 0.065M tris_Na Citrate, 0.1M NH4 acetate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |