3DS7
Structure of an RNA-2'-deoxyguanosine complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-11-12 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 |
Unit cell lengths | 35.139, 41.830, 64.810 |
Unit cell angles | 86.55, 81.16, 89.64 |
Refinement procedure
Resolution | 19.500 - 1.850 |
R-factor | 0.205 |
Rwork | 0.200 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB 1U8D |
RMSD bond length | 0.021 |
RMSD bond angle | 2.000 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | CNS |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.500 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.075 | 0.297 |
Number of reflections | 29177 | |
<I/σ(I)> | 8.4 | 3.4 |
Completeness [%] | 94.0 | 91.4 |
Redundancy | 3.56 | 3.57 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.01M K-HEPES, 0.0119 M cobalt(III) hexammine, 22% PEG 2000, 0.66M ammonium acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | K-HEPES | ||
10 | 1 | 2 | H2O | ||
2 | 1 | 1 | cobalt(III) hexammine | ||
3 | 1 | 1 | PEG 2000 | ||
4 | 1 | 1 | ammonium acetate | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | K-HEPES | ||
7 | 1 | 2 | cobalt(III) hexammine | ||
8 | 1 | 2 | PEG 2000 | ||
9 | 1 | 2 | ammonium acetate |