3DRY
X-ray crystal structure of human KCTD5 protein crystallized in low-salt buffer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97915 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 102.990, 106.791, 110.174 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.300 |
R-factor | 0.26018 |
Rwork | 0.253 |
R-free | 0.30791 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3drx |
RMSD bond length | 0.012 |
RMSD bond angle | 1.276 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.074 | 0.510 |
Number of reflections | 20180 | |
<I/σ(I)> | 18.14 | 1.9 |
Completeness [%] | 99.5 | 97.6 |
Redundancy | 5.2 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 0.2 M proline, 100 mM HEPES, 7% (w/v) PEG 3350, pH 7.5, VAPOR DIFFUSION |