3DRX
X-ray crystal structure of human KCTD5 protein crystallized in high-salt buffer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97936 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.862, 128.583, 152.524 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.110 |
| R-factor | 0.23 |
| Rwork | 0.227 |
| R-free | 0.27500 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.046 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.4.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.066 | 0.460 |
| Number of reflections | 26106 | |
| <I/σ(I)> | 15.62 | 1.5 |
| Completeness [%] | 99.1 | 99.3 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 3.2 M NaCl, 100 mM Tris-HCl, pH 8.5, VAPOR DIFFUSION, temperature 298K |
