3DRJ
Crystal structure of Lactococcal OppA co-crystallized with pTH-related peptide in an open conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-22 |
| Detector | ADSC Q210 |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.140, 123.356, 59.704 |
| Unit cell angles | 90.00, 103.91, 90.00 |
Refinement procedure
| Resolution | 37.160 - 1.500 |
| R-factor | 0.165 |
| Rwork | 0.162 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3drf |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.563 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 37.165 | 1.590 | |
| High resolution limit [Å] | 1.500 | 4.480 | 1.500 |
| Rmerge | 0.033 | 0.022 | 0.191 |
| Number of reflections | 86038 | 3250 | 13711 |
| <I/σ(I)> | 17.78 | 37 | 5.1 |
| Completeness [%] | 95.5 | 94.8 | 94.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2M NaCl, 0.1M Na-Hepes, 20% PEG 6000, pH 7.0, vapor diffusion, hanging drop, temperature 293K |
