3DR7
GDP-perosamine synthase from Caulobacter crescentus with bound GDP-3-deoxyperosamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-28 |
| Detector | BRUKER PROTEUM |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.414, 152.229, 105.754 |
| Unit cell angles | 90.00, 101.83, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| Rwork | 0.197 |
| R-free | 0.26300 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3bn1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.100 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 161560 | |
| <I/σ(I)> | 10.7 | 2.3 |
| Completeness [%] | 94.6 | 83.2 |
| Redundancy | 3.73 | 1.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 298 | 50 mM MES, 10% PEG 8000, 1 mM PLP, 1 mM glutamate, pH 6.5, batch, temperature 298K |
