3DR5
Crystal structure of the Q8NRD3_CORGL protein from Corynebacterium glutamicum. Northeast Structural Genomics Consortium target CgR117.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-22 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97945 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 50.511, 50.511, 303.330 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.300 - 2.250 |
R-factor | 0.211 |
Rwork | 0.211 |
R-free | 0.23100 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXDE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.087 | 0.283 |
Number of reflections | 19601 | |
<I/σ(I)> | 22.3 | 5.6 |
Completeness [%] | 93.4 | 69.2 |
Redundancy | 20.1 | 19.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.15 | 291 | 10% PEG 4000, 0.1M magnesium chloride, 0.1M MES, pH6.15, VAPOR DIFFUSION, HANGING DROP, temperature 291K |