3DR0
Structure of reduced cytochrome c6 from Synechococcus sp. PCC 7002
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-21 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8170 |
| Spacegroup name | P 32 |
| Unit cell lengths | 82.879, 82.879, 28.277 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.580 - 1.230 |
| R-factor | 0.10771 |
| Rwork | 0.107 |
| R-free | 0.13771 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1CTJ |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.687 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0039) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.800 | 1.260 |
| High resolution limit [Å] | 1.220 | 1.220 |
| Rmerge | 0.075 | 0.373 |
| Number of reflections | 62908 | |
| <I/σ(I)> | 25.3 | 2.4 |
| Completeness [%] | 97.2 | 73.4 |
| Redundancy | 5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 292 | 10mM HEPES, 2.2M Ammonium Sulfate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
