3DQQ
The crystal structure of the putative tRNA synthase from Salmonella typhimurium LT2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 254.541, 254.541, 94.459 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 127.000 - 2.700 |
R-factor | 0.18948 |
Rwork | 0.188 |
R-free | 0.21048 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.718 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 127.000 | 2.771 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.158 | 0.760 |
Number of reflections | 47157 | |
<I/σ(I)> | 25.71 | 2.5 |
Completeness [%] | 1.0 | 0.9956 |
Redundancy | 25.1 | 22.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.1 M Bis/Tris, 3M NaCl, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |